Our paper on developmental gene expression and assembly of the genome of the New World screwworm fly was published today in Communications Biology. It was a great collaborative effort involving scientists from the USDA-ARS, U. of Cincinnati, U. New Mexico, U. Kentucky, U. of São Paulo and NIH.
Four people have joined the lab over the past year. Dr. Aki Yamamoto has for many years studied Drosophila melanogaster genetics. He is working on D. suzukii projects, including male-only strains. Alexis Kriete and Sarah Hudadoff recently completed the first year of the Entomology and Genetics PhD programs respectively. Alexis will work on the screwworm male-only project while Sarah plans to develop X-shredder strains in Lucilia cuprina. Most recently Nicholas Pistacchio joined the lab to maintain transgenic Lucilia lines and support the screwworm male-only project. Welcome all!
Our paper on a simple killer-rescue gene drive has been published in the Proceedings of the Royal Society B. The experimental was done by Sophia Webster, a former PhD student in the lab and the modeling by Michael Vella, a PhD student in Biomathematics. This is a simple self-limiting gene drive which should be easy to adapt to other species, including disease vectors.
We previously developed a female-specific tTA overexpression system for L. cuprina and C. hominivorax. We found that in some lines females with one copy of the transgene showed high survival but all females died with two copies. This suggested that relatively small changes in the level of tTA expression could significantly impact female survival. In this recently published study, we examined the impact of standing genetic variation on female survival. We used the DGRP resource, a collection of 205 highly bred Drosophila lines. We found a wide variation in female survival in different genetic backgrounds. Thus it will be important to test a transgenic strain against insects collected from the targeted location.
We describe improved transgenic embryonic sexing strains for the Australian sheep blowfly in a recent article in Molecular Genetics and Genomics . We found that promoters from the L. cuprina nullo or Cochliomyia macellaria CG14427 genes drove high levels of tTA expression in the early embryo but had little activity at later stages. Production of tTA in embryos activated expression of the hid gene which lead to female lethality.
Our paper on gene editing in screwworm and blowflies has been published in G3. We describe our efforts to optimize CRISPR/Cas9-based gene editing through targeting the brown body gene, which is an ortholog of Drosophila yellow. We then use the method to knockout the screwworm transformer gene, which is essential for female development.
We are seeking a postdoctoral fellow to work on New World screwworm male-only strains in Pacora, Panama. See listing on our “join us” page.
Our manuscript showing that the no blokes gene is essential for male viability and X chromosome gene expression in the Australian sheep blowfly has been published in Current Biology. The paper can be freely accessed for the next month using the link: https://authors.elsevier.com/a/1XFCR3QW8RmV8V
At a conference in Raleigh in February 2016, we presented a paper on the potential of Cas9-mediated gene drive systems for control of agricultural insect pests. At the meeting a draft manuscript was made available to attendees that described in more detail the ideas outlined in the presentation. The manuscript was subsequently revised, edited and is now available on line at the Journal of Responsible Innovation.